Posters Abstracts

I. Semen Freezing Technology

A Lustyková, S Frydrychová, J Čeřovský, J Lipenský, M Rozkot

Institute of Animal Science, Prague Uhříněves, Workplace Kostelec nad Orlicí, Czech Republic

P2: Boar spermatozoa survive freezing after Single Layer Centrifugation with Androcoll-P

MFP van Wienen^1,2, M Wallgren³, JM Parlevliet², JM Morrell¹

¹Division of Reproduction, SLU, Uppsala, Sweden; ²Department of Farm Animal Health, Utrecht University, The Netherlands; 3 Quality Genetics, Hörby, Sweden

P3: Sublethal stress treatment of boar semen before cryopreservation enhances cryo-tolerance - Solutions for treatment protocols

A Horváth¹, L Végh², O Szenci¹, C Pribenszky¹

¹Veterinary Faculty, Budapest, Hungary; ²Cryo-Innovation Inc. Budapest, Hungary

P4: Individual boar variations in frozen-thawed semen supplemented with different OEP concentrations

L Fraser, E Jasiewicz, L Zasiadczyk, W Kordan

Department of Animal Biochemistry and Biotechnology University of Warmia and Mazury in Olsztyn, Poland

P5: Tacrine can significantly improve motility of frozen-thawed boar spermatozoa

CH Hu, XW Wang, SS Lu, M Zhang, YQ Lu, XG Yang, KH Lu

State Key Laboratory for Conservation and Utilization of Agricultural Bioresource in the Subtropics; Animal Reproduction Institute, Guangxi University, Nanning, Guangxi 530004, China

P6: Effect of different types of sugar in the freezing extender on the qualities of cryopreserved boar semen

P Chanapiwat¹, K Kaeoket², P Tummaruk¹

¹Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand.  ²Semen Laboratory, Department of Clinical Science and Public Health, Faculty of Veterinary Science, Mahidol University, Nakorn-Pathom, Thailand.

P7: Freezing procedure can result in more serious apoptosis of boar spermatozoa than bull spermatozoa

CH Hu, XJ Zhuang, YM Wei, M Zhang, SS Lu, YQ Lu, XG Yang, KH Lu

State Key Laboratory for Conservation and Utilization of Agricultural Bioresource in the Subtropics; Animal Reproduction Institute, Guangxi University, Nanning, Guangxi 530004, China

P8: The heat-shock protein family: are all members good predictors of the ejaculate freezability in boars?

S Sancho, I Casas, I Vilagran, M Briz, M Yeste, A Fàbrega, S Bonet

TechnoSperm (Biotechnology of Animal and Human Reproduction), Scientific and Technologic Park of the University of Girona, Girona, Spain

P9: Boar sperm from good freezability ejaculates maintain inner Cu/ZnSOD levels for up to six hours after thawing

I Casas, S Sancho, M Briz, E Sánchez, A Fàbrega, M Puigmulé, S Bonet

TechnoSperm (Biotechnology of Animal and Human Reproduction), Scientific and Technologic Park of the University of Girona, Girona, Spain

P10: Role of glutathione and procaine to stabilize the protamine/DNA complex after freezing and thawing of boar spermatozoa

M Yeste, E Estrada, I Casas, S Bonet, JE Rodríguez-Gil

Department of Animal Medicine and Surgery, School of Veterinary Medicine, Autonomous University of Barcelona, Spain

P11: DNA fragmentation after boar sperm preservation by heat-drying or freezing without cryoprotectant

U Rungroekrit, E Podhajsky, S Meinecke-Tillmann

Institute of Reproductive Biology, University of Veterinary Medicine Hannover, Germany

P12: Evaluation of Piau swine breed semen submitted to three freezing protocols

MHC Barros¹, HH Shiomi², SEF Guimarães^3*, LS Amorim², PS Lopes³, JB Siqueira⁴, RO Pinho³, AY Chaya², LHS Bulos², JD Guimarães^2*

¹Perdigão Company, Brazil; ²Veterinary Medicine Department of Federal University of Viçosa, Brazil; ³Animal Science Department of Federal University of Viçosa, Brazil; ⁴Veterinary Medicine Department of Federal University of Espírito Santo, Brazil

P13: Influence of seasonality on the thawed boar semen under tropical conditions

NM Velloso¹, GA Monteiro¹, JA Dell’Aqua Jr¹, SCOR Vexenat¹, FO Papa¹, DJ Moura², E Oba¹

¹Faculty of Veterinary Medicine, UNESP, Botucatu, Brazil;² Faculty of Agricultural Engeneering, UNICAMP, Brazil.

II. Liquid Semen Preservation

P14: Effect of dilution temperature on boar sperm quality

A López Rodríguez, T Rijsselaere, P Vyt, A Van Soom, D Maes

Faculty of Veterinary Medicine, Ghent, Belgium

P15: Effect of semen processing procedures on quality of cooled boar spermatozoa

TAA Khalifa¹, IA Tsakmakidis², IA Michos², CA Rekkas³

¹EquiBiotech-Research Services in Animal Breeding, Greece; ²Clinic of Farm Animals, School of Veterinary Medicine, Aristotle University of Thessaloniki, Greece; ³NAGREF, Veterinary Research Institute-Thessaloniki, Greece

P16: Cooling of concentrated spermatozoa for boar semen transport: Effects on sperm viability and sow fertility

AP Siqueira¹, JM Silva Filho¹, MS Palhares¹, NGM Palhares¹, GS Machado², MNP Moraes³, R Rossi¹, DV Alkmin¹

¹Department of Clinical Science, Veterinary School, Minas Gerais, Brazil; ²Integrall Brazil; ³DB-Dan Bred Brazil

P17: Resistance of liquid preserved boar spermatozoa to temporarily chilling

KF Weitze, X Le Thi, A Lübcke, D Waberski

Unit for Reproductive Medicine – Clinic for Pigs and Small Ruminants, University of Veterinary Medicine, Hannover, Germany

P18: Changes in responsiveness to capacitating stimuli in chilled boar spermatozoa in vitro

S Schmid¹, H Henning¹, AM Petrunkina², D Waberski¹

¹Unit for Reproductive Medicine, University of Veterinary Medicine, Hannover, Germany, ²CIMR, University of Cambridge, UK

P19: Supplementation of refrigerating boar semen extenders with antioxidants

M Solórzano, L Gil, C Malo

Department of Animal Pathology, Obstetrics and Reproduction Area, Faculty of Veterinary Medicine, Universidad de Zaragoza, Spain

P20: Influence of ejaculate portion and preservation method on the in vitro characteristics of boar spermatozoa

DV Alkmin¹, JM Silva Filho¹,MS Palhares¹, AP Siqueira¹, GS Machado², MNP Moraes³, NPG Motta¹

¹Department of Clinical Science, Veterinary School, Minas Gerais, Brazil; ²Integrall Brazil; ³DB-Dan Bred Brazil

P21: Influence of seminal plasma and extender on the quality of highly diluted boar semen

KF Weitze, X Le Thi, D Waberski

Unit for Reproductive Medicine – Clinic for Pigs and Small Ruminants, University of Veterinary Medicine, Hannover, Germany

P22: The effect of a plant protein component of boar semen extender on apoptotic-like changes in the spermatozoa

M Trzcińska, M Bryła, Z Smorąg

National Research Institute of Animal Production, Department of Animal Reproduction Biotechnology, Balice, Poland

P23: Improving boar semen quality by polyunsaturated fatty acids feed additive

N Am-in¹, RN Kirkwood², M Techakumphu¹, W Tantasuparuk¹

¹Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand. ²Department of Large Animal Clinical Sciences, Michigan State University, MI, USA.

P24: The effect of volume and sperm number on the quality of extended boar semen after long-term storage at 17ºC

D Martín-Hidalgo, MJ Bragado, LJ García-Marín, MC Gil

SINTREP, Veterinary Faculty, Cáceres, Spain

P25: Characteristic of acrosome integrity in boar semen extended in medium term and long term extenders and stored for 10 days at 18 ºC

K Kaeoket, P Chanapiwat

Semen Laboratory, Department of Clinical Science and Public Health, Faculty of Veterinary Science, Mahidol University, Salaya, Phuttamonthon, Nakorn-pathom 73170, Thailand

P26: Apoptotic-like changes in the spermatozoa of fresh and stored boar semen and the quality of embryos produced in vivo

M Bryla, M Trzcińska, Z Smorąg

National Research Institute of Animal Production, Department of Animal Reproduction Biotechnology, Balice, Poland

P27: Seasonal changes in boar sperm DNA fragmentation

M Bochenek, B Szczesniak-Fabianczyk,

Dept. Animal Reproduction Biotechnology, National Research Institute Animal Production, Balice, Poland

III. Semen Quality Assessment and Semen Preparation

P28: Introducing a novel CASA System for semen analysis

H Schmidt¹, P Manthey², S Diez-Presedo¹ , T May¹, G Kamp¹

¹AMP-Lab GmbH, Mainz, Germany; ²Delphi EPM, Mainz, Germany

P29: Effect of the frame rate and the number of frames per capture on boar sperm CASA analysis

A Echegaray, A Akourki, R Berges, JR Garulo

Laboratory of Biotechnology HUMECO, Huesca, Spain

P30: Leja-4 chambers toxic effect on boar sperm motility depends on manufacture date

A Echegaray, A Akourki, R Berges, JR Garulo

Laboratory of Biotechnology HUMECO, Huesca, Spain

P31: Boar sperm plasma membrane integrity assessed by Eosin-Nigrosine and SYBR14/PI: a comparison

C Garzon-Perez, A Medrano

Facultad de Estudios Superiores – Cuautitlan, Universidad Nacional Autonoma de Mexico

P32: Assessment of the fertilizing ability of trehalose /cryopreserved boar sperm by the Dual HOST/BBC assay

O Gutiérrez-Pérez^1,2, ML Juárez-Mosqueda³, S Uribe-Carvajal⁴, ME Trujillo-Ortega³

Instituto Nacional de Pediatría. ²Universidad del Valle de México.³Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México.⁴IFC, Universidad Nacional Autónoma de México. México DF, México.

P33: Measurement of intracellular calcium dynamics with Fluo-4 AM in frozen-thawed boar sperm by flow cytometry

R Fernández-Gago¹, F Martínez-Pastor², R González-Urdiales³, D Martín¹, B Alegre³, JC Domínguez¹

¹Animal Reproduction and Obstetrics²ITRA-ULE, INDEGSAL and Molecular Biology, University of León, Spain³CENTROTEC, Campo de Villavidel, Leon, Spain

P34: Incidence of sperm with cytoplasmic droplets in boar ejaculates is associated with reduced responsiveness to bicarbonate in vitro

H Henning, A Lübcke, A Shevchenko, D Waberski

Unit for Reproductive Medicine – Clinic for Pigs and Small Ruminants, University of Veterinary Medicine, Hannover, Germany

P35: The relationship between sperm nuclear shape and boar fertility using Fourier harmonic analysis

KL Willenburg¹, AM Williams², KJ Rozeboom¹, JJ Parrish³

¹ReproQuest, Inc, Fitchburg, WI, USA; ²PIC North America, Hendersonville, TN, USA; ³University of Wisconsin-Madison, Madison, WI, USA

P36: Single Layer Centrifugation with Androcoll-P can be scaled-up into 500 mL tubes

M Wallgren¹ , JM Morrell²

¹Quality Genetics, Hörby, Sweden; ²Division of Reproduction, SLU, Uppsala, Sweden.

P37: Fertility of boar spermatozoa prepared by single layer centrifugation in in vitro fertilization (IVF)

YCB Brandt, J Morrell, R González

Department of Clinical Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden

IV Artificial Insemination

P38: Recommended procedures of insemination of sows and their probable consequences

M Sviben

Freelance consultant Zagreb, Croatia

P39: Fertility of sows after AI with extended semen from two portions of boar ejaculate, stored at 5 or 17°C

DV Alkmin¹, JM Silva Filho¹,MS Palhares¹, AP Siqueira¹, GS Machado², CLA Silva¹, V Fortes³

¹Department of Clinical Science, Veterinary School, Minas Gerais, Brazil; ²Integrall Brazil; ³DB-Dan Bred Brazil

P40: Whey Proteins in the commercial extender TRIXCell + improve the Performance of Boar Semen: Prolonged Storage Capabilities and Higher Litter Size

BM van den Berg

Barex Biochemical Products, Enkhuizen, The Netherlands

P41: Implications of dose volume and sperm concentration in pig post-cervical artificial insemination

R Mozo-Martín, L Gil

Veterinary Faculty, Zaragoza, Spain

P42: Seminal plasma and spermatozoa modulate gene expression in the porcine uterus

S Junge¹, U Taylor¹, HJ Schuberth², J Guenther³, U Baulain¹, D Rath¹

¹Institute of Farm Animal Genetics, FLI, Mariensee, Germany

²Institute of Immunology, University of Veterinary Medicine, Hanover, Germany

V Bacterial Contamination of Boar Semen

P43: Bacterial and fungal burden in fresh and diluted boar semen

Ş Ciornei¹, L Runceanu¹, D Drugociu¹, P Roşca¹, M Mareş¹, G Agape², G. Nacu¹

¹Veterinary Faculty, Iaşi, Romania; ²Suinprod Roman, Romania

P44: Bacterial contamination of boar semen and its effect of sperm quality parameters during conservation

Y Dahmani, R Ausejo, JL Ubeda

Magapor SL. Parque Científico Tecnológico Agroalimentario, Ejea de los Caballeros (Zaragoza) Spain

P45: Antibiotic effects on boar sperm motility and movement characteristics

Y Dahmani, R Ausejo, JL Ubeda

Magapor SL. Parque Científico Tecnológico Agroalimentario, Ejea de los Caballeros (Zaragoza) Spain

P46: Bacterial contamination of boar semen and antibiotic sensitivities

R Ausejo, Y Dahmani, JL Ubeda

Magapor SL. Parque Científico Tecnológico Agroalimentario, Ejea de los Caballeros (Zaragoza) Spain

P47: Antibio-treatment of boar semen infected in vitro with bacteria: time and concentration dependence of antibiotic action

R Ausejo, Y Dahmani, JL Ubeda

Magapor SL. Parque científico tecnológico agroalimentario, Ejea de los Caballeros (Zaragoza) Spain

P48: Variation on PCV2 - DNA shedding in boars’ continuous ejaculates and semen quality in PRRS (-) farms in Venezuela

V Bermúdez¹, MVA Morales¹, A Tonelli², MA Escalona², O Moleiro², MJ Moreno³, T Opriessnig¹.

Universidad Central de Venezuela - Facultad de Ciencias Veterinarias, Dept. Patología Veterinaria¹, Iowa State University, Vet. Diag. Lab¹ USA, Private Practice², Schering & Plough-Intervet-Merial³.

P49: Dicol® : New concept of bacterial contamination control in boar semen

Y Dahmani, R Ausejo, JL Ubeda

Magapor SL. Parque Científico Tecnológico Agroalimentario, Ejea de los Caballeros (Zaragoza) Spain

P50: Decoupling dosing of antibiotics from degree of boar semen dilution: A necessity?

M Schulze¹, M Grobbel², K Ruediger¹, M Jung¹

¹Institute for Reproduction of Farm Animals, Schoenow; ²Leibniz Institute for Zoo and Wildlife Research, Berlin, Germany

VI Genetic Aspects

P51: Improving fertility through reciprocal translocation identification in pigs

A Williams¹, C Gladney², D McLaren², A Mileham²

¹PIC North America, Hendersonville, TN; ²Genus plc, DeForest, WI, USA

P52: Effect of parity and lactational body weight loss on reproductive performance of a modern sow genotype

APG Mellagi¹, A Panzardi¹, T Bierhals¹, NB Gheller¹, ML Bernardi², I Wentz¹, FP Bortolozzo¹

¹Setor de Suínos, Faculdade de Veterinária, UFRGS, Rio Grande do Sul, Brazil; ²Faculdade de Agronomia, UFRGS, Rio Grande do Sul, Brazil

VII Sexed Boar Semen

P53: Sex-preselected piglets derived from surgical artificial insemination with sexed sperm

YQ Zeng, XG Yang, YQ Lu, HY Xu, SS Lu, M Zhang, KH Lu

State Key Laboratory for Conservation and Utilization of Agricultural Bioresource in the Subtropics; Animal Reproduction Institute, Guangxi University, Nanning, China

P54: DNA fragmentation in sex-sorted, frozen-thawed boar sperm

D del Olmo, I Parrilla, T Tarantini, J Roca, EA Martinez, JM Vazquez
Dept. of Medicine & Animal Surgery. University of Murcia, Spain